Peptide Storage

Peptide Glossary

Peptide Storage

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Ensuring Peptide Integrity Through Proper Storage

Proper storage of peptides is pivotal in maintaining their integrity and ensuring the validity of laboratory results. Adhering to best practices for peptide storage not only preserves their stability but also prevents contamination, oxidation, and degradation, which could compromise research.

Initial Storage Steps Post-Receipt

Upon receiving peptides, immediate storage in a cold and light-averse environment is crucial. For short-term usage (days to months), refrigeration at temperatures below 4°C (39°F) is typically sufficient. Lyophilized peptides exhibit stability at room temperature for several weeks, making this an appropriate short-term storage option.

Long-Term Peptide Storage Recommendations

For storage extending several months to years, freezing at -80°C (-112°F) is recommended. This method significantly enhances peptide stability over extended periods. However, it’s vital to avoid frequent freeze-thaw cycles and storage in frost-free freezers, as these can lead to temperature fluctuations and increased degradation risks.

Preventing Oxidation and Moisture Contamination

To minimize moisture and air contamination:

– Allow peptides to reach room temperature before opening to prevent moisture condensation.

– Keep peptide containers tightly sealed and limit air exposure.

– Use dry, inert gases like nitrogen or argon for resealing to reduce oxidation, especially for peptides containing cysteine (C), methionine (M), and tryptophan (W).

– Aliquot peptides based on experimental requirements to avoid repeated freeze-thaw cycles and excessive air exposure.

Storing Peptides in Solution

Storing peptides in solution is less ideal than lyophilized storage due to reduced shelf life and increased vulnerability to bacterial degradation. Peptides with sequences including Cys, Met, Trp, Asp, Gln, and N-terminal Glu are particularly susceptible. If necessary:

– Use sterile buffers at pH 5-6 for solution preparation.

– Aliquot solutions to minimize freeze-thaw cycles.

– Store peptide solutions refrigerated at 4°C (39°F) for up to 30 days, with unstable peptides preferably frozen when not in use.

Appropriate Containers for Peptide Storage

Selecting the right storage containers is crucial:

– Containers should be clean, clear, and in good condition.

– Both glass and plastic (polystyrene and polypropylene) vials are used, with polypropylene offering chemical resistance.

– Transfer peptides from shipping containers to suitable storage vials if needed.

General Guidelines for Peptide Storage

When storing peptides, adhere to these key practices:

– Store in a cool, dry, and dark environment.

– Avoid repeated freeze-thaw cycles.

– Minimize air and light exposure.

– Refrain from long-term storage in solution.

– Aliquot peptides as per experimental needs to maintain stability.

Following these guidelines ensures that peptides remain stable and reliable for use in various laboratory experiments, thus preserving the quality and integrity of scientific research.

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